Bioaffinity Chromatography/Affinity chromatography  

Bioaffinity Chromatography process relies on the property of biologically active substances to form stable, specific, and reversible complexes. The formation of these complexes involves the participation of common molecular forces such as the Van der Waals interaction, electrostatic interaction, dipole-dipole interaction, hydrophobic interaction, and the hydrogen bond. An efficient, biospecific bond is formed by a simultaneous and concerted action of several of these forces in the complementary binding sites.

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HPLC Column | Agilent ZORBAX Carbohydrate Analysis Small Molecule Separations LC Column  

ZORBAX Carbohydrate Analysis Columns are reproducible, efficient, and flexible. These columns use ZORBAX porous silica microsphere technology. Silica manufacture, bonding and packing are all performed in our ISO9001 facilities. ZORBAX Carbohydrate Analysis Columns can handle high volume injections as much as 50 µL on a 4.6 x 150 mm column.
Features Of Agilent ZORBAX Carbohydrate Analysis Small Molecule Separations LC Column:
Reproducible
Efficient – uses ZORBAX porous silica microsphere technology; silica manufacturing, bonding and packing are all performed in Agilent's ISO 9001 facilities
Flexible – can handle high volume injections – as much as 50 μL on a 4.6 x 150 mm column
Recommended for use with refractive index detectors (RID)

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Waters Alliance HPLC  

Waters Alliance HPLC THAT WORKS AS HARD AS YOU DO 
Confidence in your results comes with high performance, day-to-day reliability, and overall system robustness. The Alliance® HPLC System was designed to meet demanding method criteria for repeatability, linearity, and accuracy, resulting in data that you can depend on with eacy analysis. Achieving low carryover with a programmable wash routine, delivering reproducible gradients with automatic solvent compressibility and Auto•Blend™  Technology, and streamlining daily start-up with SystemPREP are just some of the ways that the Alliance HPLC System delivers the performance your methods require, making your laboratory more efficient.
Waters Alliance HPLC Providing versatile, dependable performance for analytical labs, the Alliance H

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Agilent 1260 Infinity II Variable Wavelength LC Detector  

Sensitive, fast and even more analyte information with dual wavelength capabilities
The Agilent 1260 Infinity II Variable Wavelength Detector offers lowest baseline noise and drift, resulting in lowest detection limits for robust quantification of trace level components. Even more sample information can be acquired in dual-wavelength mode. Time-programmable wavelength switching provides optimum sensitivity and selectivity for your applications.Highest productivity can be achieved with fast analysis at up to 120 Hz data rates.
Features :
Robust quantification of trace level components -lowest baseline noise and drift results in lowest detection limits.
More analyte information per run- withdual-wavelength capabilities.
High-resolution gainin fast LC -at up to 120 Hz data acquisition r

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Agilent 1260 Infinity II Variable Wavelength LC Detector  

Sensitive, fast and even more analyte information with dual wavelength capabilities
The Agilent 1260 Infinity II Variable Wavelength Detector offers lowest baseline noise and drift, resulting in lowest detection limits for robust quantification of trace level components. Even more sample information can be acquired in dual-wavelength mode. Time-programmable wavelength switching provides optimum sensitivity and selectivity for your applications.Highest productivity can be achieved with fast analysis at up to 120 Hz data rates.
Features Of Agilent 1260 Infinity II Variable Wavelength Detector:
Robust quantification of trace level components -lowest baseline noise and drift results in lowest detection limits.
More analyte information per run- withdual-wavelength capabilities.
High-resoluti

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Waters ACQUITY Arc HPLC and UHPLC System  

HPLC and UHPLC method compatibility at the flip of a switch

The ACQUITY Arc System is a quaternary-based, modern LC system for scientists working with established methods who are looking for the versatility and robustness required to bridge the gap between HPLC and UPLC while continuing to support validated assays.

True plug-and-play method compatibility and the ability to switch between HPLC and UHPLC separations at the flip of a switch with Arc Multi-flow path Technology
Get mass detection, without changing the way you work, with the ACQUITY QDa Detector
Deploy the benefits of 2.x μm particle columns to boost the chromatographic performance of your methods
On-demand mobile phase creation with Auto●Blend Plus Technology, eliminating the cumbersome and error-prone appr

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Shimadzu Evaporative Light Scattering LC Detector LTII (ELSD-LTII)  

In the history of high-performance liquid chromatographs, which dates to the early 1960s, refractive index detectors (RI detectors) have often been used as general-purpose detectors. RI detectors enable the detection of components that do not possess UV absorbance and give a proportional relationship between the heights of detected peaks and the quantities of detected components. So, in comparison with absorbance detectors (UV detectors), they offer advantages such as the ability to ascertain unknown component quantities and obtain molecular weight distributions for macromolecules. On the other hand, they also have various disadvantages. For example, they cannot be used for gradient analysis, the baselines they produce are susceptible to the influence of fluctuations in the ambie

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principle of hplc:High Performance Liquid Chromatography (HPLC)  

High performance liquid chromatography (HPLC) has become a very versatile and powerful separation and analytical method over the years. It is an advanced form of liquid chromatography (LC).
Instead of introducing the solvent into the column and allowing it to drip down under the influence of gravity, in HPLC the sample is forced through the column under high pressures of nearly 400 atm, resulting in faster and more efficient separation.
This technique is also called high pressure liquid chromatography.

The Principle of HPLC
HPLC follows the same basic principle as chromatography. Different components in the sample have varying affinities to the adsorbent material. This causes a difference in the flow rate for each component which leads to their separation as they come out of the column.

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